• 专利附录
  • 专利说明
  • 权利要求
  • 类似技术
  • 同族专利
  • 引用文献
  • 法律状态
  • 优先权
    • 专利摘要:
    The invention relates to the field of biological and bioorganic chemistry, in particular, to methods for producing a means for visual indication of cholesterol on the skin surface. To obtain a means for visually indicating the cholesterol substance (nystatin, streptolysin 0, cholesterol oxidase), cross-linking substances (peroxide, alkaline phosphate - pelvic, a -chymotrypsin, (3 -galactosidase) with the help of a bifunctional cross-linking agent - meta-p-tolsulfonate N-cyclohexyl-N - {2-mss-phenol- {4) -ethyl-carbodimimide.
    公开号:SU1675770A1
    申请号:SU4357046K
    申请日:1988-01-19
    公开日:1991-09-07
    发明作者:Юрий Михайлович Лопухин；Ирина Павловна Андрианова；Виктор Викторович Зуевский；Александр Борисович Рабовский
    申请人:Научно-исследовательский институт физико-химической медицины；
    IPC主号:
    专利说明:

    The invention relates to biological and bioorganic chemistry, in particular, to methods for producing means for indicating cholesterol.
    The goal is to obtain a means for visual indication of cholesterol on the surface of the skin.
    Example 1.10 mg of horseradish peroxidase is dissolved in 1 ml of acetate buffer with a pH of 4.8. 1.5 mg of meta-p-toluenesulfonate-M-cyclohexyl-N-2-morpholinyl (4) -ethyl-carbodiimide is added. The solution is incubated for 4 hours at 4 ° C. 3 mg of nystatin is added. The resulting mixture is incubated for 12 hours at 4 ° C.
    Example 2. 1 mg of cholesterol oxidase is dissolved in 2 ml of acetate buffer with a pH of 4.8 and 3 mg of meta-p-toluenesulfonate-M-cyclohexyl-M - {2-morpholinyl (4) -ethyl} -carbodiimide is added. The solution is incubated for 6 hours at 4 ° C and 1 mg of alkaline phosphatase is added. The resulting mixture was incubated for 24 hours at 4 ° C.
    Example 3. 5 mg of streptolysin O is dissolved in 1 ml of acetate buffer with pH
    4.8 and 2 mg of meta-p-toluenesulfonate-M-cyclohexyl-N - {2-morpholinyl- {4) - methyl-carbodiimide is added. The solution is incubated for 8 hours at 4 ° C, 5 mg / 3-galactosidase is added and incubated for 18 hours at 4 ° C.
    权利要求:
    Claims (1)
    [1]
    Invention Formula
    The method of obtaining a means for visual indication of cholesterol on the skin surface by incubating a detecting substance selected from the series: nystatin, streptolysin 0, cholesterol oxidase, or a visualizing substance selected from the series: peroxidase, alkaline phosphatase, 2-chymotrypsin and ft -galactosidase, at a concentration of 2-10 mg / ml for 4-8 hours with N-cyclohexyl-N-2-morpholinyl- (4} -ethyl-3-carbodiimide meta-p-toluenesulfonate at a concentration of 1.5-3 mg / ml, followed by respectively the detecting or imaging substance in ontsentratsii 1-5 mg / ml and incubating for 12-24 hours at 4 ° C.
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    类似技术:
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    同族专利:
    公开号 | 公开日
    EP0338189A3|1990-05-02|
    AU615709B2|1991-10-10|
    FI890266A|1989-07-20|
    CA1335968C|1995-06-20|
    HUT56973A|1991-10-28|
    DD289604A5|1991-05-02|
    CS274496B2|1991-04-11|
    SU1675769A1|1991-09-07|
    AU2857889A|1989-07-20|
    PL277263A1|1989-09-04|
    FI890266A0|1989-01-18|
    DE58909664D1|1996-05-30|
    CS34889A2|1990-08-14|
    EP0338189B1|1996-04-24|
    CN1048925A|1991-01-30|
    EP0338189A2|1989-10-25|
    JPH01289498A|1989-11-21|
    AT137336T|1996-05-15|
    引用文献:
    公开号 | 申请日 | 公开日 | 申请人 | 专利标题
    US4071020A|1976-06-03|1978-01-31|Xienta, Inc.|Apparatus and methods for performing in-vivo measurements of enzyme activity|
    US4401122A|1979-08-02|1983-08-30|Children's Hospital Medical Center|Cutaneous methods of measuring body substances|
    US4458686A|1979-08-02|1984-07-10|Children's Hospital Medical Center|Cutaneous methods of measuring body substances|
    EP0118543A1|1982-09-01|1984-09-19|University Of Southern California|Substituted n-benzenesulfonyloxyphthalimides|US5489510A|1988-01-19|1996-02-06|2860601 Canada Inc.|Method for visual indication of cholesterol on skin surface agents used therefor and methods for producing such agents|
    RU94044169A|1994-12-16|1996-10-20|И.А. Кочетов|Multiple analytical member|
    LT4121B|1995-06-01|1997-03-25|Akcine Bendrove Biofa|Kit for the cholesterol vizualization on the skin and process for preparing conjugate for use in cholesterol determination|
    RU2130189C1|1997-02-20|1999-05-10|Александр Сергеевич Парфенов|Method for determining tissular cholesterol in skin|
    CA2465427A1|2004-04-28|2005-10-28|Imi International Medical Innovations Inc.|Direct assay of cholesterol in skin samples removed by tape stripping|
    CN100564539C|2004-12-31|2009-12-02|浙江伊利康生物技术有限公司|The mensuration reagent and the preparation method of cholesterol in the high-density lipoprotein |
    CN104181312B|2014-08-08|2016-06-01|安徽易康达光电科技有限公司|A kind of skin free cholesterol noninvasive detection device for atherosclerosis risk assessment|
    法律状态:
    优先权:
    申请号 | 申请日 | 专利标题
    SU884357046A|SU1675769A1|1988-01-19|1988-01-19|Method for preparation of agent, used for visual detection of cholesterol on the skin surface|
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